Describe in detail restriction endonucleases, their recognition sequences in DNA, and their application in the Southern blot and hybridization technique for diagnosis of human genetic disease. [BIOL 2030] Explain and illustrate the fundamental biochemistry required in the application of modern techniques of molecular biology: (i) screen complementary (cDNA) and genomic libraries to identify and clone specific DNA (gene) sequences; (ii) DNA sequencing [BIOL 2030] Recall the polymerase chain reaction (PCR), a key method in molecular biology. Use and know when to make use of common biological research tools such as compound microscopes, gel electrophoresis units, pipettors and micropipettors, bioinformatics tools, and enzyme assays. [BIOL 1010] [BIOL 1011] [BIOL 1030]
BIOL 3042
